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. 2014 Jun 16;9(6):e99887. doi: 10.1371/journal.pone.0099887

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© 2014 Godinho et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Mouse DCEK cells were transfected with the indicated plasmids. After 48-Gag antibody, followed by incubation with texas-red anti-mouse IgG. Colocalization with Golgi compartments was analyzed by staining with a rat anti-mouse golgi gp125, followed by incubation with a FITC-conjugated goat-anti-rat IgG. Colocalization with endogenous LAMP was analyzed by staining with a rat anti-mouse LAMP-2, followed by incubation with a FITC-conjugated goat-anti-rat IgG. Data is representative of four independent experiments.