Figure 8. Enhanced immune response induced by LAMP/gag immunization is dependent on CD4⁺ T cells.

Balb/c mice were treated or not with anti-CD4 antibody and immunized with LAMP/gag, as described in Material and Methods. Mice were also immunized with pITR vector or gag_N, as controls. Fifteen days after the second immunization, total splenocytes were cultured with CD8-restricted gag peptide and cytokine production was analyzed. A) TNF-α secretion was analyzed by ELISA. B) The cells were incubated with PercP-anti-CD8, PE-anti-CCR7 and FITC-anti-IFN-γ and the expression of CCR7 and IFN-γ among CD8⁺ cells were analyzed by FACS. The data are representative of two independent experiments. * p<0,05.