Table 3. In silico (BLAST) search for primer sequences from F. virguliforme in the IGS sequences of other Fusarium spp. and other fungi expected in fields with corn and soybean production.
| Fungal species | Disease; plant part | GenBank Numbera | Blast resultb |
| Fusarium virguliforme | AY220212c | 48.1 (Y, 100%) | |
| Corn | |||
| Fusarium culmorum | corn kernels | AJ854659c | 18.3 (Y) |
| AY102602d | 24.3 (NN) | ||
| Fusarium oxysporum | corn kernels | JQ585778c | 22.3 (Y) |
| Fusarium proliferatum | corn ear rots | GU737458c | 20.3 (Y) |
| Fusarium subglutinans | ear and kernel rot | HQ165883c | 20.3 (Y) |
| Fusarium verticillioides | ear and kernel rot | AJ880006c | 18.3 (Y) |
| Gibberella moniliforme | grain mycoflora | HQ165881c | 18.3 (Y) |
| DQ924537d | 24.3 (N) | ||
| Gibberella zeae | corn kernels | HQ165900c | 16.4 (Y) |
| XM_383254d | 26.3 (N) | ||
| Macrophomina phaseolina | Nodal root fragments | N/A | N/A |
| Rhizopus sp. | Nodal root fragments | N/A | N/A |
| Soybean | |||
| Calonectria crotalariae | Red crown rot | N/A | N/A |
| Diaporthe phaseolorum var. caulivora | Stem canker | HM769322c | 14.4 (N) |
| Fusarium solani | Fusarium root rot | JN041209d | 20.3 (N) |
| Macrophomina phaseolina | Charcoal rot | GU046842d | 24.3 (N) |
| Phialophora gregata | Brown stem rot | AF249313c | 14.4 (N) |
| AF306933d | 18.3 (N) | ||
| Rhizoctonia solani | Rhizoctonia disease | GU934565d | 22.3 (N) |
| Sclerotinia sclerotiorum | Sclerotinia stem rot | EF152636c | 24.3 (N) |
| XM_001585495d | 32.2 (N) | ||
| Thielaviopsis basicola | Thielaviopsis root rot | AY997789c | 14.4 (N) |
| GQ131878d | 18.3 (N) |
A GenBank number was provided if the search provided a reference.
The blast search was conducted if a GenBank entry was found. The highest value of the maximum score is given.
“(Y)” The 3′ end of the forward and reverse primers at least matches 5 bases of the shown sequences separately, which means the possible success of amplification for the sequence in the database by Fv-IGS primer.
“(N)” The 3′ end of the forward and reverse primers does not match at least 5 bases of the shown sequences separately, which means very low possibility of successful amplification for the sequence in the database by Fv-IGS primer.
“(NN)” The possible amplification size is too big (>5000 bp).
The hit sequences were in IGS region.
The hit sequences were not in IGS region, and they were in other nuclear regions.
“N/A” not applicable because no matches were deposited in GenBank.