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. 2014 Jun 16;9(6):e100271. doi: 10.1371/journal.pone.0100271

Figure 7. ARHGAP22 suppresses cell spreading.

Figure 7

(A) A7 cells were transfected with HA-ARHGAP22 constructs (WT, ΔGAP, R211A, or ΔCC,) and serum-starved for 20 h. Quiescent cells were trypsinized and then plated on collagen-coated coverslips and fixed 20 min after plating. The cells were stained with anti-HA antibody for HA-ARHGAP22 (green) and phalloidin for F-actin (red). Merged fluorescent images are shown. The cells were also stained with hoechst 33258 for nuclei (blue). Arrowheads indicate the transfected cells. Scale bar, 20 µm. (B) The surface area of spreading cells (n = 100) 20 min after plating were calculated and shown as box and whisker plots. **, p<0.01. Statistical significance was determined by Welch's t-test.