Figure 5. MSL and NSL bind to shared and specific gene sets.
(A) Venn Diagram showing the overlap between Msl1 and Nsl1 binding sites at ENSEMBL genes. For statistical analyses see Figure 5—figure supplement 1A–C. Binding at TSSs and gene bodies was considered together. Genes are listed in Figure 2—source data 2. (B) Gene ontology analysis using Manteia (Tassy and Pourquie, 2013) of only Msl1 binding sites, only Nsl1 binding sites, or common binding sites. Significant GO terms for only Msl1 binding sites are highlighted by a box. (C) Differentially expressed genes were identified with the DEseq analysis (Anders and Huber, 2010). The table represents genes expressed only in mESCs (when compared to NPCs). The overlap with all Msl1 positive, all Nsl1 positive or only complex specific genes was calculated. Statistical analyses in Figure 5—figure supplement 2A–D indicates significant enrichment of Msl1 at mESC-specific genes. (D and E) Bound genes were divided into three categories: Common (category 1), specific for Nsl1 (category 2) and specific for Msl1 (category 3). From these categories genes were chosen for ChIP-qPCR using the anti-Msl1 (D) or anti-Nsl1 (E) antibodies. Fold enrichment higher than five was defined as specific binding and Msl1 and Nsl1 presence was analysed at the indicated genes in each category. Bar charts represent the mean and standard deviation of 2–3 independent experiments.
Figure 5—figure supplement 1. MSL and NSL significantly bind to shared and specific gene sets.
Figure 5—figure supplement 2. MSL, but not NSL locates to mESC-specific genes.
