Skip to main content
. 2014 Jun 2;5:3935. doi: 10.1038/ncomms4935

Figure 6. Evaluation of differentiation into tissue-specific lineages and structural maturation of cells.

Figure 6

Comparative gene expression analysis for (a) chondrogenic (SOX9 and COl2A1), (b) cardiogenic (Myh6 and Actn1) and (c) adipogenic (PPARγ and LPL) in COL and particular dECM (cdECM or hdECM or adECM) at day 14. Immunofluorescence images showing chondrogenic differentiation of hTMSCs in (d) COL and (e) cdECM constructs showing COL type II staining (COLII, red), cell nuclei (DAPI, blue) and F-actin (green) (scale bar, 50 μm). Structural maturation of myoblasts in (f) COL and (g) hdECM construct showing Myh7 (red) and cell nuclei (DAPI, blue) (scale bar, 200 μm). Adipogenic differentiation of hASCs in (h) COL and (i) adECM constructs showing PPARγ (red), COL IV (green) and cell nuclei (DAPI, blue) (scale bar, 50 μm). All experiments were performed in triplicate. Error bars represent s.d. (*P<0.05).