Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jun 6;5:4054. doi: 10.1038/ncomms5054

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

PMC Copyright notice

Immunofluorescent staining (IMF) for podoplanin (Pdpn; green) in the intact adult epicardium identifies clusters of cells within basement membrane (white arrowheads; a–c). Clusters in the epicardium (white arrowheads) are encapsulated by ECM as indicated by IMF for fibronectin (Fn; green; d). Inset box in d is shown at higher magnification in e, and CD29 (β1-integrin; f). IMF for CD44 and CD45 revealed the clusters were a heterogeneous population of mesenchymal (CD44; g) and HCs (CD45; h). The clusters resided in close proximity to coronary vessels (i,j) as confirmed by immunostaining on a Tie2Cre;R26R-tdTomato background (k). cv, coronary vessel; ep, epicardium; my, myocardium. Scale bars, 40 μm (a); 20 μm (b–e); 10 μm (f–k).