Table 5.
Summary of mRNA expression of genes of interest using RT-PCR
| Chr | GeneID | Coding Length | Fold Change | 95% CI | P Value |
|---|---|---|---|---|---|
| GENES IDENTIFIED IN RNA-SEQ AS SIGNIFICANT OR BORDERLINE | |||||
| Neurotransmitter-related genes | |||||
| chrX | P2RY4 | 1639 | 2.714 | (0.94, 4.49) | 0.024 |
| chr6 | VIP | 1586 | 0.949 | (0.00001, 1.94) | 0.433 |
| Ion-channel function-related genes | |||||
| chr1 | GUCA2B | 597 | 2.046 | (0.60, 3.50) | 0.061 |
| chr11 | PDZD3 | 2363 | 1.492 | (0.72, 2.27) | 0.217 |
| Tissue repair, structural proteins, and barrier genes | |||||
| chr21 | TFF1 | 492 | 1.616 | (0.41, 2.82) | 0.514 |
| chr3 | RBP2 | 688 | 1.699 | (0.58, 2.82) | 0.056 |
| chr2 | FN1 | 8975 | 0.417 | (0.21, 0.62) | 0.012 |
| Interferon-related genes | |||||
| chr10 | IFIT3 | 2688 | 0.447 | (0.11, 0.78) | 0.209 |
| Chemokine-related genes | |||||
| chr2 | CCL20 | 842 | 0.654 | (0.00001, 1.45) | 0.637 |
| chr1 | C4BPA | 2243 | 2.398 | (0.19, 4.61) | 0.040 |
| OTHER GENES OF INTEREST MEASURED BY RT-PCR | |||||
| Tight junction protein-related genes | |||||
| 3 | CLDN1 | 3481 | 0.5123 | (0.24, 0.78) | 0.10 |
| 5 | OCLN | 6438 | 1.0341 | (0.48, 1.59) | 0.76 |
| 15 | ZO-1 | 7950 | 0.7658 | (0.45, 1.09) | 0.35 |
| Bile acid homeostasis-, receptor-, and absorption-related genes | |||||
| 5 | FGFR4 | 3122 | 0.8182 | (0.61, 1.03) | 0.17 |
| 2 | GPBAR1 | 2003 | 0.8386 | (0.56, 1.12) | 0.34 |
| 12 | FXR/NH1R4 | 2884 | 1.7404 | (0.94, 2.54) | 0.038 |
| 13 | IBAT/ASBT | 3777 | 1.7337 | (0.28, 3.19) | 0.09 |
| Serotonin transporter gene | |||||
| 17 | SLC6A4 | 6543 | 0.9596 | (0.20, 1.72) | 0.87 |
| Immune function-related gene | |||||
| 9 | TNFSF15 | 6687 | 0.4718 | (0.16, 0.79) | 0.057 |
P values in italics reflect borderline significance, and those in bold reflect significance.