Fig. 4.

The Abcg2-lineage increases after oxidative stress and is vascular. A: gene expression of lineage-sorted cardiac cells treated with PQ compared with control. eNOS3, endothelial nitric oxide synthase (NOS3); vWF, von Willebrand factor; Tagln, transgelin; Tnnt2, troponin T type 2. B: immunohistochemical comparison of endothelial markers [CD31 (a and b) and vWF (c and d)], smooth muscle marker [smooth muscle myosin (SMM); e and f], and cardiac muscle [α-sarcomeric actinin (a-actinin); g and h]. Arrowheads denote coexpression of ZsGreen and labeled marker. C: quantification of coexpression normalized to total nuclei. Scale bars = 50 μm. *P < 0.05.