Fig. 4.
PNx induces increased nuclei numbers in α1+/− mice. Left ventricle sections from each experimental group were stained with 4′,6-diamidino-2-phenylindole (DAPI; blue) and cardiac troponin I (cTnI, red) as described in materials and methods. A: representative images of DAPI and cTnI staining. Scale bar = 25 μm. B: quantification data of nuclei number by counting the DAPI-stained particles that overlaid with cTnI staining. C: quantification data for nuclei that are outside of the cTnI staining area. *P < 0.05, PNx vs. sham in α1+/− animals.