Fig. 5.
PNx induces apoptosis in α1+/− mice but not in WT mice. Active caspase-3 levels in mouse heart tissue were measured using immunohistochemical methods as described in materials and methods. A, left: representative ×20 photomicrographs of active caspase 3 in sham- or PNx-operated animals. A, right: quantification data from 5 to 6 animals in each group. B, left, representative terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) images from sham- and PNx-operated animals. B, right: quantification of TUNEL-positive nuclei. Red arrows indicate TUNEL-positive nuclei. WT sham, n = 6; WT PNx, n = 5; α1+/− sham, n = 6; PNx+/−, n = 6. *P < 0.05, significantly different than sham; !significantly different than WT PNx. Scale bar is 60 μm.