Fig. 6.

PNx activates the mammalian target of rapamycin pathway in WT mice but not in α₁^+/− mice. Heart tissue homogenates from experimental mice were analyzed for Na/K-ATPase α₁ (NKA)-content, phosphorylation of Akt (pAkt) at threonine-308, pAkt at serine-473, and phosphorylation of S6 ribosomal protein (pS6) using Western blot analysis. Top, right: Western blots. tAkt, total Akt. A–D: respective quantification data of Na/K-ATPase α₁ (A), pS6 (B), pAkt Thr308 (C), and pAkt Ser473 (D) taken from 8 animals in each group. *P < 0.05, significant difference vs. WT control; !P < 0.05, PNx α₁^+/− significantly different than sham α₁^+/−; #P < 0.01, significant difference between PNx WT and PNx α₁^+/−.