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. 2014 May 6;306(12):E1449–E1459. doi: 10.1152/ajpendo.00663.2013

Fig. 5.

Fig. 5.

ATGL/T372D loses LD localization in HeLa cells. A: HeLa cells transfected with ATGL/S47A, ATGL/S47A/T372D, or ATGL/S47A/T372A were incubated under growth conditions with 400 μM oleic acid for 16 h. Immunofluorescence staining with anti-Myc (red) antibody was performed. LDs were costained with Bodipy 493/503 dye (green). B: relative intensity of red immunofluorescence (anti-Myc) on LD surface and cytosol in cells transfected with different Myc-ATGL variants. Data are shown as means ± SD and represent 4 independent cell images. ***P < 0.001. C: relative fluorescent intensity of Bodipy per cell in cells transfected with different Myc-ATGL variants. Data are shown as means ± SD and represent 3 independent images.