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. 2014 Apr 29;306(12):E1418–E1430. doi: 10.1152/ajpendo.00048.2014

Fig. 7.

Fig. 7.

Intracerebroventricular gAPN acts in the hypothalamus. A: immunohistochemistry showing induction of Fos-immunoreactive cells (dark purple) in coronal sections of arcuate nucleus. Scale bar, 20 μm. B–E: CB1 (B), TPH2 (C), CART (D), and 5-hydroxytryptamine (serotonin) receptor 2C (Htr2C; E) hypothalamic gene expressions following infusion in WT mice. F and G: changes in cerebrospinal fluid (CSF; F) and serum APN concentration (G) following infusion in WT mice. H: changes in serum APN concentration following infusion in APN-KO mice. H–K: CB1 (I), TPH2 (J), and Htr2C (K) quantitative RT-PCR analysis following gAPN treatment in mHypoE-N1 cells. L: APPL1 and TPH2 protein expression in gAPN-induced mHypoE-N1 cells. M–O: CB1 (M), TPH2 (N), CART (O), and Htr2C (P) quantitative RT-PCR analysis following gAPN treatment with or without pretreatment with APPL1 shRNA lentivirus in mHypoE-N1 cells. All quantitative RT-PCR was normalized to β-actin mRNA expression. All data are means ± SD; n = 5 mice. *P < 0.05 as determined by independent-samples t-test.