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. 2014 Jun 17;4:78. doi: 10.3389/fcimb.2014.00078

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Copyright © 2014 Miko, Rivas, Bentancor, Delannoy, Fach and Beutin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Alignment of the amino acid sequences of the C-terminal ends of the A-subunits and B-subunits of 8 stx2d-positive and 8 stx2d-negative O178:H19 strains investigated in this study. Amino acid sequences in bold show the activatable tail in the A-subunit and the END motif in the B-subunit. Amino acid sequences which contain both motifs, thus correspond to the mucus-activatable stx2d variant, are shaded. The following sequences were used as references: stx2a (GenBank accession no. X07865), stx2b (AF043627), stx2c (M59432), and stx2d (AF479829). Amino acid sequences underlined indicate double peaks in the chromatogram which reveal multiple stx2-toxin types in these strains. ^aPosition in mature toxin sequence.