Figure 3. Effect of the NOS inhibitor L-NAME on arachidonic acid metabolism by rat cerebral arteries.

Arteries were incubated with ¹⁴C-AA and indomethacin in the absence (A) and presence of L-NAME (B). Metabolites were extracted and separated by reverse-phase HPLC, solvent system 1. Migration times of known standards are shown on each chromatogram. CPM, counts per minute; THETA, trihydroxyeicosatrienoic acid; HEETA, hydroxyepoxyeicosatrienoic acid; HETE, hydroxyeicosatetraenoic acid; AA, arachidonic acid.