Table 1. Primer and locus information.
Diagnostic nuclear loci (dSNP) that provided at least a 90% probability of distinguishing between the parental species are starred.
| Locus | dSNP | Freq. of dSNP in LA | Forward primer (5′-3′) | Reverse primer (5′-3′) | Length (bp) | %GC | % Identical sites | D BF | D LA |
|---|---|---|---|---|---|---|---|---|---|
| cyt-b | – | – | TTTGCCCTATCTATCCT | GATCCTGTTTCGTGGAGGAAGGT | 609 | 48 | 97.7 | −1.51 | NA |
| MHC* | 1 | 1.0 | CCGGCAGCAGTACGTGCACTTCGNACAGCGA | GATGGGCTGCTGCAGGCTGGTGTGCT | 571 | 63.5 | 99.1 | −0.22 | −1.28 |
| 1FWD* | 2 | 0.90 | GTGCCACCCATGTAACACCT | TGTGCTTTGGATGAACAGTTG | 429 | 55 | 99.5 | NA | −0.26 |
| 1REV* | 3, 4, 5 | 1.0 | ACTGTGTCACCCCATGCTC | CTGAGTCATTTCCATTCCTGG | 407 | 58.7 | 99.0 | −0.87 | NA |
| 4FWD* | 6 | 1.0 | TGGGCCAGGTTGTTAGGTAG | TATTGGTGGAATGGGCTTGT | 464 | 34.3 | 99.4 | −1.16 | NA |
| 4REV* | 7 | 1.0 | GGCTGGGGGTTTGGAATTA | CTTTCTACAGAGAAATAAACAAAGACC | 443 | 36.9 | 99.5 | −0.24 | NA |
| 6FWD | – | – | AGGGGTCTCTCAAACAGCAA | CTGGCCCTTTAGATAATAGCC | 418 | 35.8 | 99.8 | 1.53 | NA |
| 6REV | – | – | GAAGCGTAGTGAAGTATAACATCGTG | ATGCTGAGGGTGCCATCTTA | 458 | 39.5 | 98.9 | 0.47 | −1.76 |
| 10FWD | – | – | GGCAAAGGCTAAAGGCAAAG | TCAGAATTATTATAGCTTCAGGTGAG | 548 | 43.4 | 99.6 | NA | 0.06 |
| 10REV | – | – | GGTGGTAGAACAGAAAGTCT | TTACCACCTTCCACCACACA | 495 | 36.2 | 99.6 | 0.87 | NA |
Notes.
Tajima’s D of NA indicates no variation occurring at that locus.
- BF
- Black-footed Albatross
- LA
- Laysan Albatross