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. Author manuscript; available in PMC: 2014 Jun 17.
Published in final edited form as: Proteomics. 2010 Jan;10(1):141–158. doi: 10.1002/pmic.200900195

Figure 3.

Figure 3

ATP content, viability, and mitochondrial membrane potential of untreated and LPA-treated C13NJ microglia. (A) ATP content of cells incubated in the presence of LPA (2 μM) for the indicated times. ATP levels were analyzed by HPLC as described in Section 2. Results represent mean±SD from triplicate dishes. p<0.001, p<0.05 versus baseline. (B) Mitochondrial activity of cells was analyzed using the MTT assay. Cells were incubated in the presence of LPA (2 μM) for the indicated times and processed as described in Section 2. Results represent mean±SD (n = 4). (C) Measurement of mitochondrial membrane potential by JC-1 staining assay. Cells were cultured on 96-well plates and incubated in the presence of the indicated LPA concentrations for 1 or 5 h. Staining with JC-1 and fluorescence measurements were performed as described in Section 2. Results (fluorescence intensities) are expressed as percentage of controls (no LPA added) and shown as mean±SD (n = 6).