Table 2.
Protein identification
| Spot no. | Full protein name | Protein name | Swiss-Prot accession no. |
Mw theor. | Pl theor. | Mw exp. | pl exp. | Fold-regulation | No. of peptides matched |
Sequence coverage (%) |
|---|---|---|---|---|---|---|---|---|---|---|
| Groupl: metabolism | ||||||||||
| Upregulated | ||||||||||
| 1551, 1672, 1673,1682, 1684, 1691, 1729, 1849 | α-Enolase | ENOA | P06733 | 47 169 | 7.01 | 49 200–55 600 | 5.8–7.5 | ↑2.2–7.9 | 6–23 | 22–64 |
| 2186, 2189 | Fructose-bisphosphate aldolase A | ALDOA | P04075 | 39 420 | 8.30 | 41 200 | 8.7 | ↑2.6/2.5 | 6/7 | 19/25 |
| 2405, 2418, 2423, 2435 | Glyceraldehyde-3-phosphate dehyd rogenase | GAPDH | P04406 | 36 053 | 8.57 | 36 500 | 6.7–9.7 | ↑2.1–2.8 | 3–6 | 13–21 |
| 1433 | Pyruvate kinase isozymes M1/M2 | KPYM | P14618 | 57 937 | 7.96 | 61900 | 7.2 | ↑2.5 | 10 | 24 |
| 3013 | Triosephosphate isomerase | TPIS | P60174 | 26 669 | 6.45 | 22 700 | 7.0 | ↑2.4 | 3 | 14 |
| 2422 | l-lactate dehydrogenase B chain | LDHB | P07195 | 36 638 | 5.71 | 35800 | 5.5 | ↑5.6 | 9 | 31 |
| 1850 | Fumarate hydratase, mitochondrial | FUMH | P07954 | 54 637 | 8.85 | 49 200 | 7.1 | ↑3.9 | 6 | 17 |
| 1574 | GARS protein | Q15374 | 45 987 | 6.34 | 56000 | 7.2 | ↑5.1 | 11 | 41 | |
| 2136 | Gastric-associated differentially lexpressed protein YA61P (drug-sensitive protein 1) | Q9NZ23 | 14 867 | 6.83 | 42 700 | 5.8 | ↑2.8 | 3 | 33 | |
| Downregulated | ||||||||||
| 2184 | Cytosolic acyl coenzyme A thioester hydrolase | BACH | 000154 | 41 796 | 8.85 | 46 200 | 7.1 | ↓5.1 | 3 | 10 |
| 1038 | Guanine monophosphate synthetase variant | Q53F90 | 76 696 | 6.24 | 72 600 | 6.7 | ↓2.0 | 6 | 12 | |
| 2405 | Methionine adenosyltransferase 2 subunit β | MAT2B | Q9NZL9 | 37 552 | 6.9 | 36 400 | 6.7 | ↓4.9 | 4 | 18 |
| Group 2: cytoskeleton/motility | ||||||||||
| Upregulated | ||||||||||
| 2485 | Annexin A1 | ANXA1 | P04083 | 38 714 | 6.57 | 34 600 | 6.7 | ↑2.2 | 16 | 60 |
| 3368 | Galectin-7 | LEG7 | P47929 | 15 075 | 7.02 | 13800 | 6.9 | ↑2.1 | 3 | 30 |
| 3295 | Nucleoside diphosphate kinase B | NDKB | P22392 | 17 298 | 8.52 | 15 600 | 6.7 | ↑3.9 | 4 | 14 |
| 2148 | Twinfilin-2 | TWF2 | Q6IBS0 | 39 548 | 6.37 | 41700 | 6.3 | ↑3.7 | 5 | 24 |
| 1130 | WD repeat-containing protein 1 | WDR1 | 075083 | 66194 | 6.17 | 70000 | 6.6 | ↑2.2 | 9 | 29 |
| 2535 | WD repeat-containing protein 61 | WDR61 | Q9GZS3 | 33 581 | 5.16 | 33300 | 6.5 | ↑4.1 | 4 | 24 |
| Downregulated | ||||||||||
| 2839 | Tubulin folding cofactor B | TBCB | Q99426 | 27 326 | 5.06 | 27 000 | 5.0 | ↓2.4 | 3 | 17 |
| 1383 | Vimentin | VIME | P08670 | 53 652 | 5.06 | 60800 | 5.0 | ↓2.3 | 24 | 54 |
| Group 3: chaperones/unfolded protein response | ||||||||||
| Upregulated | ||||||||||
| 3056 | BAG family molecular chaperone regulator 2 | BAG 2 | 095816 | 23 772 | 6.25 | 22 200 | 6.5 | ↑6.4 | 6 | 30 |
| 477 | Heat shock protein 105 kDa | HS105 | Q92598 | 96 865 | 5.28 | 92 900 | 5 | ↑2.8 | 33 | 42 |
| 904 | 78kDa glucose-regulated protein (immunoglobulin heavy chain-binding protein) | GRP78 | P11021 | 72 333 | 5.07 | 80 500 | 4.7 | ↑3.3 | 22 | 43 |
| 584, 591 | Endoplasmin [Precursor] | ENPL | P14625 | 92 469 | 4.76 | 89 500 | 5.3 | ↑2.1/2.6 | 18/6 | 33/10 |
| Downregulated | ||||||||||
| 1660 | Hsp90 co-chaperone Cdc37 | CDC37 | Q16543 | 44 468 | 5.17 | 53700 | 4.6 | ↓2.1 | 6 | 22 |
| Group 4: protein binding, synthesis, modification, and secretion | ||||||||||
| Upregulated | ||||||||||
| 3227 | Calmodulin-like protein 5 | CALL5 | Q9NZT1 | 15921 | 4.34 | 17 400 | 7.9 | ↑2.4 | 5 | 35 |
| 1786 | Septin-2 | SEPT2 | Q15019 | 41 487 | 6.15 | 51 200 | 6.3 | ↑2.9 | 9 | 34 |
| 1786 | Septin-5 | SEPT5 | Q99719 | 42 111 | 6.21 | 51 200 | 6.3 | ↑2.9 | 3 | 11 |
| 2485 | Vacuolar protein sorting-associated protein 37B | VP37B | Q9H9H4 | 31 307 | 6.78 | 34 600 | 6.7 | ↑2.2 | 3 | 19 |
| 2919 | Ubiquitin carboxyl-terminal hydrolase isozyme L1 | UCHL1 | P09936 | 24 824 | 5.33 | 24700 | 5.3 | ↑2.8 | 11 | 51 |
| Downregulated | ||||||||||
| 1041 | Annexin A6 | ANXA6 | P08133 | 75 873 | 5.42 | 118900 | 7.5 | ↓2.1 | 4 | 9 |
| 2787 | Calcyclin-binding protein | Q6NVY0 | 26182 | 7.63 | 28000 | 8.6 | ↓2.5 | 7 | 36 | |
| 2270 | Calmodulin-like skin protein variant [Fragment] | Q53H37 | 15 876 | 4.34 | 39 000 | 6.7 | ↓6.3 | 3 | 19 | |
| 2692 | Proteasome activator complex subunit 3 | PSME3 | P61289 | 29 506 | 5.69 | 30000 | 5.5 | ↓2.3 | 7 | 29 |
| 2150 | Protein disulfide-isomerase [Precursor] | PDIA1 | P07237 | 57 116 | 4.76 | 41 200 | 4.7 | ↓2.0 | 5 | 12 |
| 2839 | Tumor protein D52-like 2 | Q5JWU8 | 21 450 | 5.71 | 27 000 | 5.0 | ↓2.4 | 4 | 27 | |
| Group 5: RNA synthesis, binding, turnover | ||||||||||
| Upregulated | ||||||||||
| 1679 | Lupus La protein (La autoantigen) | LA | P05455 | 46 837 | 6.68 | 53700 | 6.8 | ↑6.2 | 5 | 15 |
| 2307 | Heterogeneous nuclear ribonucleoproteins C1/C2 | HNRPC | P07910 | 33 670 | 4.95 | 37 800 | 5.3 | ↑2.7 | 5 | 23 |
| Downregulated | ||||||||||
| 2424 | Heterogeneous nuclear | ROA1 | P09651 | 38 846 | 9.26 | 35800 | 9.4 | ↓2.5 | 3 | 12 |
| ribonucleoprotein A1 | ||||||||||
| 2288 | Heterogeneous nuclear | HNRH3 | P31942 | 36 926 | 6.27 | 37 800 | 6.9 | ↓2.5 | 3 | 12 |
| ribonucleoprotein H3 | ||||||||||
| 830, 849 | Far upstream element-binding protein 2 | FUBP2 | Q92945 | 73146 | 6.84 | 80 500 | 7.0 | ↓2.1/2.2 | 13/16 | 24/27 |
| 1989 | TAR DNA-binding protein 43 | TADBP | Q13148 | 44 740 | 5.85 | 46700 | 5.6 | ↓3.7 | 8 | 29 |
Information on protein name, the Swiss-Prot accession number, theoretical Mw and pl, experimental Mw and pl, fold-regulation, number of identified peptide sequences, and % sequence coverage are included. Protein identifications are based on multiple peptide sequences with a minimum number of three distinct peptides.