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Britanin inhibits IgE/Ag-induced translocation of 5-LO and phospho-cPLA2. IgE-sensitized BMMCs were pre-incubated with britanin or Bay 61-3606 for 1 h and stimulated with DNP-HSA for 15 min. Cytosolic (A) and nuclear fractions (B) were immunoblotted with antibodies for phospho-cPLA2 (Ser505) and 5-LO. β-Actin and lamin B were used as internal controls for cytosol and nuclear fractions, respectively.
