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Effects of britanin on phosphorylation of Syk, LAT and PLCγ1. IgE-sensitized BMMCs were stimulated with or without DNP-HSA for 5 min. Proteins were immunoprecipitated for Syk or LAT to assess their phosphorylation statuses. Phosphorylation of PLCγ1 was determined using total cell lysates. Blots are representative of three independent experiments that provided similar results.
