Fig. 2.

Knock-down of zfGrx2 leads to cardiac looping failure. In situ hybridization detecting transcripts of the cardiac-myocyte specific gene cmlc2 revealed that embryos lacking zfGrx2 suffer from heart looping defects at 48 hpf. Whereas the angle between atrium and ventricle in wildtype embryos was 54 ± 17° (n = 29), we measured an angle of 112 ± 24° in zfGrx2 morpholino injected embryos (n = 35). Cardiac looping could be restored to an angle of 89 ± 21° when zfGrx2 capped mRNA was injected simultaneously with the morpholino (n = 27). A: Atrium, V: ventricle. Presented are mean ± SD (^***p < 0.0005).