Fig. 5.

Knock-down of glutaredoxin 2 induces cell death in the areas of formation and migration of neural crest cells. Using acridine orange (AO) staining in 19 somite stage embryos, we detected cell death in the area of specification and migration of NC cells in zfGrx2 knock-down embryos, whereas wildtype controls showed only few acridine orange positive cells. Apoptotic cell death was confirmed by TUNEL staining at 24 hpf. Detecting neural crest cells using the specific marker foxD3 shows less staining rostral to the otic vesicle as well as in the rostral brain of embryos lacking zfGrx2 compared to wildtypes, indicating loss of NC cells. Asterisks mark the otic vesicle.