Skip to main content
. 2014 May 5;155(7):2578–2588. doi: 10.1210/en.2013-2030

Figure 4.

Figure 4.

Changes in the circadian expression of metabolic and clock genes in POMC neurons in the hypothalamus of adult EGFP-POMC mice given a diet of milk formula containing ethanol (AF) or pair-fed isocaloric diet (PF) or left in the litter with the mother (AD) during the postnatal period. A–H, Daily rhythms in the mRNA levels of Pomc (A), metabolic-related genes (Stat3, B; Sirt, C; Pgc1a, D; Asb4, E), and clock genes (Bmal1, F; Per1, G; Per2, H). Relative mRNA levels of various genes were calculated as in Figure 1. *,**,***, Significantly different (P < .05, P < .01, P < .001, respectively) between means at different time points among the PF and AF groups from the lowest value, as per a one-way ANOVA with the Dunnett posttest. a, aa, aaa, Significant difference (P < .05, P < .01, P < .001, respectively) between AF and PF as per a two-way ANOVA with the Bonferroni post hoc test. Black bar represents the dark period. Data are mean ± SEM of five to six animals per time point.