Figure 1.

Serum LH and estradiol in diestrous, OVX, and OVX + E₂-treated mice. Mice were OVX at 2 months of age and treated with sc capsules containing either E₂ or vehicle. Blood samples were taken at 3 months of age. A, Serum LH was significantly increased in OVX mice, compared with diestrous and E₂-treated OVX mice. Similar patterns of LH secretion were observed in wild-type and Tac2-EGFG mice. B, Treatment of OVX Tac2-EGFP mice with E₂ capsules resulted in diestrous levels of serum E₂. n = 5–10 mice/group. *, significantly different from diestrous and OVX + E₂ mice (within wild-type and Tac2-EGFP groups, ANOVA with Tukey's post hoc test).