Figure 7.

Evoked activity of arcuate Tac2-EGFP neurons in OVX and OVX + E₂ mice. A, Representative patterns of activity evoked by a series of rectangular-pulse current injections. A tonic pattern was identified with little frequency adaptation (top), and a phasic pattern was characterized by a few spikes and rapid adaptation (middle). B, Proportions of neurons identified as tonic or phasic in OVX and OVX + E₂ mice. A greater percentage of tonic neurons was detected in the OVX mice, but these changes were not statistically significant (Fisher's exact test). C, In response to depolarizing current injections, OVX + E₂ mice exhibited reduced spike frequencies, compared with OVX mice (mean ± SEM; OVX, n = 16 neurons from 9 mice; OVX + E₂, n = 15 neurons from 11 mice). *, significantly different, OVX vs OVX + E₂ (two-way ANOVA with Tukey's post hoc tests).