Figure 2.
mRNA expression of adipogenic genes in PA vs C adult female rhesus monkeys. Subcutaneous abdominal adipose tissue collected from 6 PA and 6 C was immediately placed into liquid nitrogen after animals were killed. mRNA extraction and qRT-PCR were performed as indicated in Materials and Methods. Zfp423 (A), PPARδ (B), CEBPα (C), and PPARγ (D) mRNA levels were quantified by qRT-PCR relative to the housekeeping gene GAPDH. Results represent fold changes in PA (n = 6) vs C (n = 6) monkeys; *, P < .020; **, P < .001 vs C.