Table 2.
Frequency of CD4+ T cells and CD4 + CD25 +/high CD127 low/- regulatory T cells in the peripheral blood and synovial fluid and synovial membrane joint samples in rheumatoid arthritis and osteoarthritis patients a
| RA | OA |
P
-values |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| RA:OA |
RA |
RA |
RA |
OA |
OA |
OA |
||||
| PB:SF | PB:SM | SF:SM | PB:SF | PB:SM | SF:SM | |||||
| PB |
Sample size (n) |
18 |
22 |
|
|
|
|
|
|
|
| Sample volume (ml) |
7 ± 0 |
7 ± 0 |
|
|
|
|
|
|
|
|
| Concentration of CD4+ T cells (cells /μl) |
730 ± 210 |
690 ± 90 |
0.424 |
<0.0001b |
<0.0001b |
|
<0.0001b |
<0.0001b |
|
|
| (610 to 900) |
(540 to770) |
|||||||||
| CD4+CD25high (%total CD4+ T cells) |
4.88 ± 2.04 |
4.4 ± 1.59 |
0.417 |
0.0072b |
0.0381b |
|
<0.0001b |
0.004b |
|
|
| (2 to 9.4) |
(1.9 to 8) |
|||||||||
| CD4+CD25+/highCD127low/- (% total CD4+ T cells) |
6.7 ± 1.8 |
7.6 ± 1.8 |
0.135 |
0.0006b |
0.0128b |
|
0.0001b |
0.0091b |
|
|
| (4 to 11) |
(5 to 11) |
|||||||||
| CD4+CD25+/highCD127low/- (cells/μl) |
48.9 ± 7.8 |
52.4 ± 4 |
0.099 |
<0.0001b |
0.0004b |
|
<0.0001b |
<0.0001b |
|
|
| (30 to 99) |
(29 to 85) |
|||||||||
| SF |
Sample size (n) |
17 |
22 |
|
|
|
|
|
|
|
| Sample volume (ml) |
15.41 ± 12.65 |
11.21 ± 6.81 |
|
|
|
|
|
|
|
|
| (4.3 to 38) |
(2.2 to 23.6) |
|||||||||
| Concentration of CD4+ T cells (cells/μl) |
70 ± 170 |
40 ± 90 |
0.479 |
<0.0001b |
|
0.0001b |
<0.0001b |
|
<0.0001b |
|
| (30 to 540) |
(10 to 480) |
|||||||||
| CD4+CD25high (% total CD4+ T cells) |
9.97 ± 6.27 |
9.63 ± 4.12 |
0.7371 |
0.0072b |
|
0.2449 |
<0.0001b |
|
0.1086 |
|
| (3.4 to 19) |
(3.3 to 22.6) |
|||||||||
| CD4+CD25+/highCD127low/- (% total CD4+ T cells) |
11.8 ± 5.5 |
12.4 ± 5 |
0.727 |
0.0006b |
|
0.1226 |
0.0001b |
|
0.4549 |
|
| (5 to 22) |
(4 to 26) |
|||||||||
| CD4+CD25+/highCD127low/- (cells/μl) |
8.3 ± 9.4 |
5 ± 4.5 |
0.153 |
<0.0001b |
|
<0.0001b |
<0.0001b |
|
<0.0001b |
|
| (8to90) |
(4 to 100) |
|||||||||
| SM | Sample size (n) |
18 |
22 |
|
|
|
|
|
|
|
| Sample volume (mg) |
3.45 ± 1.18 |
3.72 ± 1.3 |
|
|
|
|
|
|
|
|
| (2.2 to 5.9) |
(1.95 to 5.73) |
|||||||||
| Concentration of CD4+ T cells (cells/μg) |
378 ± 250 |
240 ± 140 |
0.0336b |
|
<0.0001b |
0.0001b |
|
<0.0001b |
<0.0001b |
|
| (10 to 610) |
(10 to 340) |
|||||||||
| CD4+CD25high (% total CD4+ T cells) |
7.21 ± 3.9 |
7.5 ± 4.8 |
0.8354 |
|
0.0381b |
0.2449 |
|
0.004b |
0.1086 |
|
| (2 to 18.4) |
(2.5 to 26) |
|||||||||
| CD4+CD25+/highCD127low/- (% total CD4+ T cells) |
9.4 ± 3.8 |
11.2 ± 5.9 |
0.266 |
|
0.0128b |
0.1226 |
|
0.0091b |
0.4549 |
|
| (5 to 17) |
(2.4 to 26) |
|||||||||
| CD4+CD25+/highCD127low/- (cells/μg) | 35.5 ± 12 |
27 ± 11 |
0.025b | 0.0004b | <0.0001b | <0.0001b | <0.0001b | |||
| (24 to 89) | (14 to 73) | |||||||||
aOA, Osteoarthritis; PB, Peripheral blood; RA, Rheumatoid arthritis; SF, Synovial fluid; SM, Synovial membrane. Collected sample volumes and calculated concentrations of CD4+ T cells are shown. Frequencies of CD4+CD25high and of CD4+CD25+/highCD127low/- cells are shown as the percentage of CD4+ T cells and for CD4+CD25+/highCD127low/- cells also as cell concentration (total cell counts per microlitre or per microgram) for PB, SF and SM in RA and OA patients. Values shown are mean ± SD (range) unless otherwise stated. bStatistically significant differences.