Table 3.
Phenotype characteristics of CD4 + CD25 +/high CD127 low/- T-cells from peripheral blood, synovial fluid and synovial membrane of rheumatoid arthritis and osteoarthritis patients a
| |
RA |
OA |
P
-values |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Surface marker | PB | SF | SM | PB | SF | SM |
RA |
OA |
PB |
SM |
| PB:SM | PB:SM | RA:OA | RA:OA | |||||||
| CD45RA |
22.9 ± 5 |
5 ± 3 |
2.8 ± 1 |
34.9 ± 13.6 |
6 ± 3 |
5.6 ± 2.5 |
0.0059b |
0.0006c |
0.0631 |
0.2377 |
| 21 (19.5 to 30.2) |
4 (3 to 7) |
2.8 (2 to 3.5) |
30.8 (16.8 to 57.4) |
7 (2 to 9) |
6 (2.1 to 8.8) |
|||||
| CD45RO |
83 ± 14.7 |
93 ± 7 |
94.7 ± 5.2 |
81.4 ± 8.5 |
92 ± 8 |
91.9 ± 7.2 |
0.4 |
0.0232b |
0.9554 |
0.6488 |
| 81 (70 to 99) |
92 (87 to 99) |
94.9 (89.4 to 99) |
82 (60 to 95) |
91 (81 to 98) |
94.3 (80.9 to 99.8) |
|
||||
| CD69 |
3.1 ± 1.9 |
88 ± 2 |
84.8 ± 0.2 |
1.9 ± 0.8 |
82.3 ± 5.3 |
81.2 ± 4.8 |
< 0.0001c |
0.0029d |
0.1623 |
0.4746 |
| 2.1 (1.9 to 5.2) |
85 (80 to 92) |
84.6 (78 to 89) |
1.8 (0.9 to 3.7) |
84 (77.3 to 88) |
82.6 (74.6 to 84.9) |
|||||
| CD62L |
94.6 ± 1.8 |
5 ± 2 |
2 ± 1 |
94.7 ± 4.4 |
3 ± 1 |
3.1 ± 0.5 |
< 0.0001c |
0.0293b |
0.8881 |
0.2480 |
| 94.5 (93.3 to 95.8) |
3 (3 to 9) |
2 (1.1 to 3.2) |
95.3 (80.5 to 99.7) |
3.5 (2 to 5) |
3.1 (2.8 to 3.5) |
|||||
| CD152 |
12.6 ± 14 |
29.2 ± 12 |
36.2 ± 3.3 |
13 ± 6.5 |
31.5 ± 11 |
32.5 ± 8.2 |
0.0477b |
0.0049d |
0.7552 |
0.8530 |
| 5.7 (3.2 to 28.8) |
33 (33 to 40) |
36 (33 to 39) |
16.3 (1 to 19) |
30 (24 to 41) |
32.5 (25 to 39.6) |
|||||
| CD154 |
13.5 ± 6.3 |
11 ± 5.7 |
15.1 ± 8.8 |
8.8 ± 5.4 |
12 ± 8 |
13.4 ± 8.2 |
0.5892 |
0.2863 |
0.2361 |
0.8486 |
| 13.4 (7 to 19.5) |
13 (6 to 18) |
15.1 (7 to 23) |
8.5 (1.4 to 19) |
11 (5 to 20) |
10 (7.5 to 18) |
|||||
| CD274 |
23.5 ± 2.8 |
25 ± 7 |
27.6 ± 5.7 |
9.8 ± 10.4 |
25 ± 7 |
24.1 ± 7.9 |
0.3758 |
0.0313b |
0.0329b |
0.5541 |
| 23 (21 to 26) |
32 (20 to 29) |
31 (21 to 31) |
6 (0.5 to 40) |
26 (17 to 34) |
24.1 (17 to 30.9) |
|||||
| CD279 |
3.3 ± 3.3 |
23.2 ± 12 |
22.6 ± 11 |
5.4 ± 6.5 |
9 ± 9 |
7.9 ± 10 |
0.0231b |
0.6677 |
0.8127 |
0.0239b |
| 3 (0.2 to 6.7) |
24 (14 to 32) |
22.6 (15 to 30.1) |
2.2 (0 to 23.1) |
8 (2 to 15) |
8 (0.8 to 15) |
|||||
| GITR | 30.3 ± 22.7 |
42.5 ± 7.3 |
45.5 ± 6.8 |
40.5 ± 12.8 |
49.2 ± 12 |
47.4 ± 11.9 |
0.329 | 0.173 | 0.636 | 0.8114 |
| 31.4 (7 to 52.4) | 43 (39 to 51) | 45.1 (38.9 to 52) | 43.8 (18.4 to 59.1) | 13.9 (33 to 59) | 11.94 (30 to 58.7) | |||||
aGITR, Glucocorticoid-induced tumour necrosis factor receptor–related protein; OA, Osteoarthritis; PB, Peripheral blood; RA, Rheumatoid arthritis; SF, Synovial fluid; SM, Synovial membrane. Significant differences: bP < 0.05; cP < 0.001; dP < 0.01. CD4+CD25+/highCD127low/- regulatory T cells from PB, SF and SM were stained for cell surface markers as indicated in the left column. The percentage of cells positive for each surface marker is presented as mean ± SD and median and range. Data are representative of three RA patients) and five OA patients. Statistical analysis is shown for PB and SM because SF values did not differ those for SM.