Figure 3.

Specific identification of CB₁ receptors in purified brain mitochondria by Western blot analyses. (A) Representative immunoblotting of CB₁ receptors from CB₁-WT and CB₁-KO brain lysates, using rabbit anti-CB₁ C-ter12 antibody. (B) Representative immunoblotting (three independent experiments) showing that CB₁ receptors are specifically immunoprecipitated from wild-type purified brain mitochondria with different CB₁ receptor antibodies. CB₁ C-ter12: primary rabbit anti-CB₁ antibody binding to a 12 amino acids sequence of the C-terminus; CB₁ N-ter14: primary rabbit anti-CB₁ antibody binding to a 14 amino acids sequence of the N-terminus; CB₁ C-ter31: primary goat anti-CB₁ antibody binding to a 31 amino acids sequence of the C-terminus. (C) Immunoblot of Slp2 from CB₁-WT and CB₁-KO brain lysates (10 and 20 μg), Tom20 used as mitochondrial loading control.