Skip to main content
. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: Nat Med. 2014 May 4;20(6):607–615. doi: 10.1038/nm.3541

Figure 1. Expression of FasL on the human tumor endothelium.

Figure 1

(a) Representative dual staining of the endothelium (CD34, Red) and FasL (brown) from normal and tumor tissues from the indicated organs. Original magnification, 400×; scale bar, 50 μm. (b) Summary of FasL vessel expression of the indicated tumor types. (c) Expression of FasL on cultured normal endothelial cells, activated T cells and two representative ovarian tumor samples gated on CD45−CD31+ endothelial cells. Shaded areas are isotype control staining, and dotted line in T cell plot is FasL from unstimulated T cells. All solid lines are FasL staining. (d) Quantification of FasL on tumor endothelial cells isolated from individuals with ovarian cancer (n = 11). (e) Representative image showing vessels from tumor (T) and surrounding stromal tissue. Original magnification 40×; scale bar, 100 μm. Enlarged vessels original magnification 200×; scale bar, 50 μm. (f) Quantification of FasL expression on the tumor endothelium of individuals with ovarian cancer. (n = 27). P value determined by a paired t test.