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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Struct Biol. 2014 Apr 26;187(1):1–9. doi: 10.1016/j.jsb.2014.04.006

Figure 4. Native enveloped Sindbis virus directly captured from infected BHK-21 cell culture supernatant for single particle cryo-EM 3D reconstruction.

Figure 4

Negative staining images show that Sindbis particles specifically bind to the grid coated with anti-E2 antibodies (B) but not to the grid without anti-E2 (A). (C) shows one representative cryo-SPIEM images of Sindbis particles directly from the 8-fold concentrated cell culture supernatant. (D) Single particle 3D reconstruction of the captured particles. “2”, “3” and “5” represent the icosahedral two-, three- and five-fold axes, respectively. (E) Fourier shell correction of the reconstruction. (F) plots the Euler angles of the Sindbis particles captured on the cryo-SPIEM grid.