The Effect of TLR9 Agonist CpG Oligodeoxynucleotides on the Intestinal Immune Response of Cobia (Rachycentron canadum)

. 2014 Jun 2;2014:273284. doi: 10.1155/2014/273284

(a) Primers used for cloning of TLR9 isoform B

Primer name	Primer sequence (5′-3′)	Application
TLR9F1^a	ATCTCAGCCACAATCAGATC	Partial cloning
TLR9R2^a	AGTTTGGGAAACATCTCATC	Partial cloning

TLR9B1 RacF^b	CTGTGAATACCCTGAGTCTCAACAGGG	RACE
TLR9B RacR^b	GTGATGCTGTGAATCAGTACCAGCC
Universal primer mix (UPM)	Long 0.2 μM CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT Short 0.4 μM-CTAATACGACTCACTATAGGGC
Nested universal primer mix (NUP)	AAGCAGTGGTATCAACGCAGAGT
TLR9B1-NGSP1	GCCTTTATGTCCTGCCCAAAGCACC
TLR9B-NGSP2	CTTCCTCGTCTGTTCCATCTTGGCTGT