(b) Primers used for gene expression studies
| Primer name | Primer sequence (5′-3′) | Product size (bp) | Target gene | Gene bank accession number | Application |
|---|---|---|---|---|---|
| B3-5c | ACAGACTGTTCCTCCTCCCC | 532 | 3′UTR | HM754627 | cDNA quality |
| B3-2c | GAAACCTCCAACAGCGGG | ||||
| RAF | AAGGACCTGTACGCCAACA | 330 | β-Actin-3 | HM754627 | |
| RB1 | TGGCGTCTCGCATCGTTT | ||||
|
| |||||
| TLR9A-RC F | TCTGTTCCATCTTGGCTGTG | 160 | TLR9A | KC180322 | qRT-PCR |
| TLR9A-RC R | CTGGTTTCTGGTGTCAAACA | ||||
| TLR9B-RC F | GCCTTCCTCGTCTGTTCCAT | 178 | TLR9B | JX073035.1 | |
| TLR9B-RC R | ACAGCCTGGTTTCTGGTGTC | ||||
| MyD88-F1Q | GAGGTGTAAGAGGATGGTGGT | 183 | MyD88 | KF018033.1* | |
| MyD88-R2Q | GGTGGGGAATGGCTTTGTCAT | ||||
| RCIL-1βF1 | CAGGCAGAACAACCACTGAC | 170 | IL-1β | AY641829 | |
| RCIL-1βR2 | TTCCAAGTCCAGTCCTTTGG | ||||
| RCMxF1 | TGGACATAGCAACCACAGAG | 157 | Mx | AY834185 | |
| RCMxR2 | TTCTTCAGGTGGATGACCTC | ||||
| RCIgM-F1 | AGACAGCCTGCAGGGAAAAG | 182 | IgM | JX025102 | |
| RCIgM-R2 | TGTTCCTTTCCCCCAGTAGT | ||||
| CC1eF | ATTACAATAAGAACCCTGTGC | 185 | CC | JF975593 | |
| CC1eR | TCTTTCCTGGGATGGATTTG | ||||
| β-Actin RC-F | ACAGACTGTTCCTCCTCCCC | 160 | β-Actin-3 | HM754627 | |
| β-Actin RC-R | AAAATCCTGAGTCAAGCGCC | ||||
aPartial sequence primers.
bTouchdown primers for 5′ and 3′ designed from TLR9 isoform B partial sequence.
cPrimers designed from 3′UTR of cobia β-actin-3.
*Partial sequence cloned in this study.