Table 4.
In vitro oxidation of VLDL-LDL isolated from control and macrosomic rats.
| Day 0 | Day 0 | Day 90 | Day 90 | |
|---|---|---|---|---|
| Control | Macrosomic | Control | Macrosomic | |
| Basal oxidation hydroperoxide (nmol/mg VLDL-LDL protein) | ||||
| ISIO | 15.29 ± 1.38 | 26.77 ± 1.05** | 27.50 ± 1.22 | 57.95 ± 2.17** |
| EPAX | 13.74 ± 1.02 | 19.94 ± 1.11∗§ | 25.95 ± 1.15 | 43.18 ± 2.08∗§§ |
| Oxidation with CuSO4 Hydroperoxide (nmol/mg VLDL-LDL protein) | ||||
| ISIO | 46.51 ± 3.42 | 83.87 ± 4.36** | 79.94 ± 5.54 | 162.52 ± 8.48** |
| EPAX | 50.36 ± 2.33 | 102.96 ± 5.25∗§ | 90.27 ± 6.38 | 296.98 ± 9.41∗∗§§ |
| Oxidation with CuSO4 TNBS (free lysine groups, %) | ||||
| ISIO | −17.25 ± 1.04 | −29.5 ± 1.26** | −30.8 ± 2.40 | −42.67 ± 2.35** |
| EPAX | −15.16 ± 1.35 | −27.6 ± 2.18** | −31.35 ± 2.32 | −45.98 ± 2.29** |
Values are mean ± SD; n = 8 rats. Day 0: birth; day 90: adulthood; EPAX: EPAX diet; LDL: low density lipoprotein; ISIO: ISIO diet; TNBS: trinitrobenzene sulfonic acid assay; VLDL: very low density lipoprotein. Data were analysed statistically by ANOVA. Significant differences between macrosomic and control rats and between ISIO and EPAX diets at each age were assessed using Student's t test. *P < 0.05 and **P < 0.01 compared with the control group. § P < 0.05 and §§ P < 0.01 compared with the group fed with the ISIO diet.