Figure 1.

Expression of ${\text{Lenti - siI}}_2^{\text{PP2A}}$ decreases ${\text{I}}_2^{\text{PP2A}}$ level in htau mice. (A) ${\text{I}}_2^{\text{PP2A}}$ (I₂) protein in the hippocampus of the ~11-month-old htau mice or the age-control wild-type (wt) mice was detected by Western blotting. (B) Approximately 11-month-old htau mice received brain infusion of the ${\text{Lenti - siI}}_2^{\text{PP2A}}$ (Lenti-siI₂) or the scrambled ${\text{Lenti - ssiI}}_2^{\text{PP2A}}$ (Lenti-ssiI₂; 2 μl each, 2 × 10⁹ TU/ml) into the hippocampal CA3 region under a stereotaxic instrument as described in the Section “Materials and Methods.” Four weeks later, the expression levels of ${\text{I}}_2^{\text{PP2A}}$ were detected by Western blotting and quantitative analysis. (C) The expression of the lentivirus in CA3 region was presented. The eGFP was stained in the cell body and dendrites of neurons. L, lateral; M, medial; sl, stratum lucidum; so, stratum oriens; sp, stratum pyramidale. The data were presented as mean ± SD of three independent experiments. **p < 0.01 vs. wt mice or Lenti-ssiI₂/htau2 (htau mice infused with Lenti-ssiI₂). Scale bar: 20 μm.