Figure 5.

Expression of ${\text{Lenti - siI}}_2^{\text{PP2A}}$ restores PP2A activity with inhibition of GSK-3 in htau mice. Approximately 11-month-old htau mice were treated as Figure 1. (A) The activity of PP2A was measured by a PP2A activity assay kit as described in the Section “Materials and Methods.” (B–D) The protein levels of the Ser9-phosphorylated GSK-3β (pS9) and total GSK-3β (tGSK-3β) (B), the levels of phosphorylated Akt at Ser473 (pS473-Akt) and total Akt (C), and the levels of PKAα, PKAIβ, and PKAIIα (D) were measured by Western blotting and quantitative analysis. The data were presented as mean ± SD of three independent experiments. *p < 0.05; **p < 0.01 vs. Lenti-ssiI₂/htau2 (htau mice infused with Lenti-ssiI₂).