Figure 4.

Acute injury increases local Klotho expression within skeletal muscle. Young (3–4 months old) wild type animals were exposed to a cardiotoxin injury to the tibialis anterior muscle. Two weeks after injury, TAs were harvested, cryosectioned and incubated with anti-Klotho (red), anti-Dystrophin (green) and nuclear (blue) antibodies (left image; 20× magnification). Corresponding high magnification image (right image; 100× magnification). Note that Klotho is undetectable in areas of more mature, regenerating myofibers. In contrast, strong expression of Klotho is observed in the area of cellular infiltrate.