FIG 10.

Pbx proteins occur in detergent-resistant membranes. Total cell lysate cleared of cell debris (S1) was subjected to ultracentrifugation, and the supernatant fraction (S2) was removed. The pellet (P2) was extracted with TX-100 or SDS and fractionated into detergent-insoluble (Insol.) and detergent-soluble (Sol.) fractions. The cell wall-containing fraction (P1) was treated with lysing enzyme and fractionated into supernatant (Sup.) and pellet fractions. The same number of cell equivalents of each fraction of the indicated strains was resolved by SDS-PAGE and immunoblotted with anti-HA antibody. See Materials and Methods for details.