FIG 4.

pbxΔ mutants are deficient in capsule shedding. Shown are the results of immunoblot analysis of CM or purified GXM from the indicated strains grown in rich medium (YPD) or CIM. Samples (50 μl of CM or 10 μg of GXM) were resolved by agarose gel electrophoresis and immunoblotted with antibody 3C2. The exposure time was 2 s (images after a longer exposure are shown in Fig. S2 in the supplemental material). The reduced migration of GXM from cells grown in CIM reflects the increased polymer size that results from capsule induction (49).