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. 2014 May 16;16(3):R113. doi: 10.1186/ar4564

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Copyright © 2014 El Mansouri et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of interleukin 1β on histone H3 lysine 4 methylation at microsomal prostaglandin E synthase 1 promoter. Osteoarthritis (OA) chondrocytes were treated with 100 pg/ml interleukin 1β (IL-1β) for the indicated time periods, and chromatin immunoprecipitation (ChIP) assays were performed using antibodies specific to mono-, di- and trimethylated histone H3 lysine 4 (H3K4). The results are expressed as fold changes relative to control (that is unstimulated cells), and the data are the mean ± SD of three independent experiments using cells from four different donors. For each ChIP assay, the immunoprecipitated DNA was quantitated in triplicate on two separate occasions. *P < 0.05 compared with unstimulated cells. me1, Monomethylation; me2, Dimethylation; me3, Trimethylation.