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. 2014 Jun 1;4:30. doi: 10.1186/2045-3701-4-30

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Copyright © 2014 De-Donatis et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Single molecule polarization detection to investigate motor rotation. (A) Experiment design of single molecule polarization detection on motor pRNA rotation during DNA packaging. The motor was stalled by γ-S-ATP and the rotation of pRNA ring can be excluded since no anti-correlated signals of a single Cy3 fluorophore in horizontal (H) or vertical (V) channels were observed. (B and C) Typical time trajectories of Cy3 fluorescence intensity in horizontal (black) and vertical (red) channels without (B) and with (C) the addition of ATP to restart the packaging.