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Pairwise variation (V) analysis for six potential reference genes of sugarcane (α-tubulin, glyceraldehyde 3 phosphate dehydrogenase, histone H1, S-adenosylmethionine decarboxylase, ubiquitin, and 25S rRNA) based on the GeNorm analysis [17]. The addition to the two most stable genes (αTUB and GAPDH) of a random third gene (V 2/3 = 0.15), a fourth gene (V 3/4 = 0.14), or even more (V 4/5 and V 5/64) still exhibited desirable values (basically below than 0.15).
