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. 2014 Jun 2;2014:357052. doi: 10.1155/2014/357052

Table 1.

Potential reference genes and target genes with the individual accession numbers, annotations, and primer sequences.

Gene (accession numbers) Source* Predicted function Description Primer sequences (forward/reverse)
Reference genes        
  αTUB (CN607271) dbEST Structural constituent of cytoskeleton Alpha-tubulin (F) CCATTGGCAAGGAGATTGTT
(R) TCCACCAACTGCATTGAAGA
 GAPDH (CA254672) dbEST Glycolysis, gluconeogenesis Glyceraldehyde 3 phosphate dehydrogenase (F) GGTTCACTTGAAGGGTGGTG
(R) TGAGGTGTACCTGTCCTCGTT
 H1 (CA116806) [23] Chromatin condensation Histone H1 (F) CGCACACGCACACTGAAAG
(R) CGGTGGCCATGATCAAAAA
 SAMDC (CA127376) [23] Polyamine and triamine biosynthesis S-Adenosylmethionine decarboxylase (F) TGCTGCTGAAGACGCTGTTG
(R) TCGCCTTCAAAGCAGTGTAGAAC
 UBQ (CA077378) dbEST Protein degradation Ubiquitin (F) ACCGAAGGTTGCATTCAAGAC
(R) GGGTTTGGGTCCGTTAGAAG
 25S rRNA (BQ536525) dbEST Translation 25S ribosomal RNA (F) GCAGCCAAGCGTTCATAG
(R) CGGCACGGTCATCAGTAG
Target genes        
 PFPα1 (XM_004973200.1) dbEST Carbohydrate metabolism, glycolysis Pyrophosphate fructose-6-phosphate 1-phosphotransferase alpha subunit (F) TTATGAGTTGCTGCGAGAGAAG
(R) TATCTCAATGTCGCCCATGTAG
 AS (FM212633.1) dbEST Asparagine synthesis Glutamine-dependent asparagine synthetase (F) CCAGAGAACACACCCACAAC
(R) ATGCCACACTAGGACCTCCA
 PIP1-1 (CF572112) dbEST Integral to membrane, water transport Plasma membrane intrinsic protein 1-1 (F) GTTCCTATCCTTGCCCCACT
(R) AGGCGTGATCCCTGTTGTAG
 ACC oxidase (TC127289) SoGI Ethylene biosynthesis 1-Aminocyclopropane-1-carboxylate oxidase (F) GGGACCTCTTGCAGATAATGTC
(R) CTCTGGCAATGGTCCATAGAA

*Databases: dbEST (NCBI; http://www.ncbi.nlm.nih.gov/) and Gene Index (SoGI; http://compbio.dfci.harvard.edu/tgi/); Rodrigues et al. [23]. αTUB: alpha-tubulin; GAPDH: glyceraldehyde 3 phosphate dehydrogenase; H1: histone H1; SAMDC: S-adenosylmethionine decarboxylase; UBQ: ubiquitin; 25S rRNA: 25S ribosomal RNA; PFPα1: pyrophosphate fructose-6-phosphate 1-phosphotransferase alpha subunit (EC 2.7.1.90); AS: glutamine-dependent asparagine synthetase (EC 6.3.5.4); PIP1-1: plasma membrane intrinsic protein; ACC oxidase: 1-aminocyclopropane-1-carboxylate oxidase (EC 1.14.17.4).