Table 2.
Parameters derived from RT-qPCR data analysis*, including PCR amplification efficiency (E) established by calibration curves for each quantified target.
| Gene | Tm (°C) | Product size (bp) | Average Cq | E (%) | NTC (Cq) | Correlation coefficient (R) | Slope | Y intercept |
|---|---|---|---|---|---|---|---|---|
| αTUB | 75.9 | 104 | 27.48 | 99.53 | 35.82 | 0.998 | −3.33 | 37.34 |
| GAPDH | 81.8 | 100 | 23.69 | 100.89 | N.D. | 0.984 | −3.30 | 41.40 |
| H1 | 78.0 | 57 | 24.76 | 97.41 | 33.59 | 0.999 | −3.39 | 37.92 |
| SAMDC | 79.0 | 60 | 22.74 | 99.66 | 36.62 | 0.992 | −3.33 | 34.36 |
| UBQ | 81.2 | 153 | 24.64 | 98.34 | N.D. | 0.999 | −3.36 | 37.86 |
| 25S rRNA | 82.9 | 108 | 14.00 | 99.82 | N.D. | 0.999 | −3.33 | 35.54 |
| PFPα1 | 83.6 | 151 | 21.92 | 92.99 | 32.90 | 0.988 | −3.50 | 40.44 |
| AS | 79.8 | 112 | 24.94 | 105.05 | N.D. | 0.990 | −3.21 | 40.26 |
| PIP1-1 | 84.6 | 134 | 24.47 | 95.55 | N.D. | 0.995 | −3.43 | 37.50 |
| ACC oxidase | 82.2 | 152 | 30.21 | 93.52 | N.D. | 0.990 | −3.49 | 44.65 |
*Based on MIQE Guidelines [26]. Tm: melting temperature (°C); N.D.: not detected; NTC: no template control; αTUB: alpha-tubulin; GAPDH: glyceraldehyde 3 phosphate dehydrogenase; H1: histone H1; UBQ: ubiquitin; SAMDC: S-adenosylmethionine decarboxylase; 25S rRNA: 25S ribosomal RNA; PFPα1: pyrophosphate fructose-6-phosphate 1-phosphotransferase alpha subunit (EC 2.7.1.90); AS: glutamine-dependent asparagine synthetase (EC 6.3.5.4); PIP1-1: plasma membrane intrinsic protein; ACC oxidase: 1-aminocyclopropane-1-carboxylate oxidase (EC 1.14.17.4).