Table 3.
Expression stability values for sugarcane candidate calculated by NormFinder and BestKeeper software.
Ranking | NormFinder analysis | BestKeeper analysis | ||
---|---|---|---|---|
Gene name | M | Gene name | CV ± SD | |
1 | αTUB | 0.11 | H1 | (1.06 ± 0.26) |
2 | H1 | 0.16 | αTUB | (1.06 ± 0.29) |
3 | GAPDH | 0.19 | GAPDH | (1.33 ± 0.31) |
4 | 25S rRNA | 0.28 | 25S rRNA | (3.89 ± 0.55) |
5 | UBQ | 0.31 | UBQ | (2.04 ± 0.50) |
6 | SAMDC | 0.33 | SAMDC | (2.53 ± 0.57) |
M: average expression stability value; CV: coefficient of variance; SD: standard deviation; αTUB: alpha-tubulin; H1: histone H1; GAPDH: glyceraldehyde 3 phosphate dehydrogenase; 25S rRNA: 25S ribosomal RNA; UBQ: ubiquitin; SAMDC: S-adenosylmethionine decarboxylase.