Table 4.
Relative expression rates of target genes (PFPα1, AS, PIP1-1, and ACC oxidase) based on RT-qPCR with roots, cDNAs of sugarcane accessions under abiotic stress, and respective unitag regulation by SuperSAGE analysis covering droughta stress (24 h of continuous dehydration) or saltb stress (100 mM NaCl).
| Unitag | Annotation | SuperSAGE [FC/Regulation*] | RT-qPCR∗& | ||
|---|---|---|---|---|---|
| Tolerant | Sensitive | Tolerant | Sensitive | ||
| SD282748a | AS | 1.92#/UR | −1.10#/ns | 1.473#/UR | 1.038#/ns |
| D179780a | PFPα1 | 1.99#/ns | −1.07#/ns | 0.756#/ns | 1.403#/ns |
| ASS122537b | ACC oxidase | 1.95/UR | — | 2.174/UR (30′) 1.830/ns (90′) |
— |
| ASS140030b | PIP1-1 | −1.31/ns | — | 0.805/ns (30′) 1.057/ns (90′) |
— |
AS: glutamine-dependent asparagine synthetase (EC 6.3.5.4); PFPα1: pyrophosphate fructose-6-phosphate 1-phosphotransferase alpha subunit (EC 2.7.1.90); ACC oxidase: 1-aminocyclopropane-1-carboxylate oxidase (EC 1.14.17.4); PIP1-1: plasma membrane intrinsic protein. #Bulk with four accessions by each library; FC: fold change [ratio of the frequencies (normalized to 1,000,000) observed in the stressed library in relation to the control library]; &relative expression level by REST software (v.2.0.13) after the ΔΔCq method, *P < 0.05 [27]; UR: upregulated; ns: not significant at P < 0.05. The time in the parentesis represents the salt stress exposition (min).