Skip to main content
. 2013 Dec 18;7(4):892–904. doi: 10.1038/mi.2013.105

Figure 6.

Figure 6

Lymphocyte retention in Salmonella-infected Peyer's patches (PPs) is independent of type I interferon receptor (IFNAR) and tumor necrosis factor (TNF)-α receptor 1. (a) Experimental setup to investigate effect of IFNAR deficiency on lymphocyte retention in PPs. Irradiated recipients were given bone marrow from CD45.1+ wild-type (wt) and CD45.2+ IFNAR−/− donor mice. Thus, generated mixed IFNARwt/ko chimeric mice were infected with Salmonella strain SL1344ΔaroA. PPs were fluorescein isothiocyanate (FITC) injected and analyzed 1 day after injection. Fluorescence-activated cell sorting (FACS) plots show the gating strategy for analysis. The left plot is gated on viable PP cells. Corresponding setups were used to investigate the effects of TNFR1 and CD69 deficiency. (b) Lymphocyte retention in PPs of infected IFNARwt/ko chimeras. PP cells were subgated on IFNARwt and IFNARko cells and percentage of FITC+ cells was determined. IFNARwt and IFNARko cells were further subgated on CD19/CD4/CD8α and analyzed for retention. Two independent experiments, total six mice per group. Mice were analyzed on day 6 or 7 of infection (‘inf'). Symbols represent individual PPs. (c) Lymphocyte retention in PPs of infected mixed TNFR1wt/ko chimeras was investigated as described in (a) and (b). Two independent experiments, total seven to eight mice per group. Dots represent individual PPs.