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. Author manuscript; available in PMC: 2014 Jun 17.
Published in final edited form as: J Immunol. 2004 May 1;172(9):5638–5647. doi: 10.4049/jimmunol.172.9.5638

Figure 6. Ad:IFN-β transduction reduces lymphocyte infiltration in the TG of HSV-1 infected mice.

Figure 6

Female C57BL/6 mice (n=4/group) were transduced with Ad Ad:IFN-β or Ad:Null vectors (1 × 106 transducing units/eye). Two days later, the mice were infected with HSV-1 (600 pfu/eye). Mice were euthanized 7 days post infection and the TGs were removed. TGs were collagenized and single cell suspensions were stained with the fluorochrome conjugated anti-CD4, anti-CD8α, anti-DX5, and anti-CD3 antibodies for 30 min at 4°C. The cells were analyzed on a FACS Calibur using WinMDI data analysis software.