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. 2014 May 27;111(23):8547–8552. doi: 10.1073/pnas.1402275111

Fig. 1.

Fig. 1.

Identification and characterization of mutants showing transgenerational transmission of the heat-stress release of TGS. (A) Bioluminescence images of the progeny (M3 and M4) of two mutant candidates (9.2.1 and 62.2.1) and of the controls (LUC25, and mom1 LUC25), all grown under control conditions at 21 °C. The two mutant lines are M3 progeny of heat stressed M2 parents recovered from the mutant screen. The green and red signals are luciferase luminescence and auto-fluorescence of chlorophyll, respectively. (B) Two new mutant alleles of the DDM1 gene (9.2.1 and 62.2.1) were identified in the screen. The ddm1-2 allele was used for the experiments presented in Fig. 2. (C) DNA methylation distribution at the ubiqutin3 promoter of the LUC transgene in 9.2.1 (M3), 62.2.1 (M3), and LUC 25 (WT). Colored and open circles represent methylated and unmethylated cytosines respectively, with red representing CG sites, blue CHG and green CHH (H can be A, T, or C). (D) Percentage of cytosine methylation in the ubiqutin3 promoter. Black bars, 9.2.1; gray bars, 62.2.1; white bars, LUC25.